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KMID : 1145120160140040359
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Asian Journal of Beauty and Cosmetology
2016 Volume.14 No. 4 p.359 ~ p.368
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Anti-bacterial and Anti-oxidant Activities of Solvent Fractions of Arecae pericarpium Extracts
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Song Seon-Young
Lee Hyun-Hwa
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Abstract
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Purpose: This present study was performed to investigate anti-oxidant and anti-bacterial activities of Arecae pericarpium MeOH extracts and its solvent fractions.
Methods: Arecae pericarpium was extracted by 80% MeOH and fractionated by n-hexane, ethyl acetate (EtOAc), n-butanol, and H2O. Free radical scavenging activities on 80% MeOH Arecae pericarpium extracts and its fractions were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Anti-oxidant effects were confirmed by catalase (CAT) and superoxide dismutase (SOD) activities. Anti-bacterial activity was evaluated by the paper disk diffusion method.
Results: Extraction yields of 80% MeOH Arecae pericarpium extracts and its fractions such as n-hexane, EtOAc, n-butanol, and H2O, were 19.04, 14.33, 12.07, 22.53, and 50.97%, respectively. The 80% MeOH extracts got the highest value (16.6 mg/g) in total polyphenol contents. In the case of DPPH free radical scavenging activity, the n-hexane fraction was 102.9 mg/mL. It was similar to the value of positive control, vitamin C and butylated hydroxytoluene (BHT). The highest values of SOD and CAT activities were confirmed at the EtOAc fraction (38.73 U mg protein-1) and the n-butanol fraction (2.75 U mg protein-1 min-1), respectively. In the results of anti-bacterial activity, 80% MeOH Arecae pericarpium extracts had anti-bacterial effects on all eight microorganisms. The n-hexane fraction had the anti-bacterial effects on only Listeria monocytogenes. There are no anti-bacterial effects on the other fractions such as EtOAc, n-butanol, and H2O.
Conclusion: It is considered that these results imply the 80% MeOH Arecae pericarpium extracts can be used as not only natural antioxidants but also natural antibacterials.
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KEYWORD
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Arecae pericarpium, Solvent fractions, Anti-oxidant activity, Anti-bacterial activity, Polyphenol
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